A Chromatographic Method for the Purification of K99 Pili from Enterotoxigenic Escherichia coli
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چکیده
منابع مشابه
A new method for the extraction and purification of K99 pili from enterotoxigenic Escherichia coli and their characterization.
It was found that K99 pili from enterotoxigenic Escherichia coli (of bovine origin) could be extracted by treatment with 3M-KSCN solution. The K99 pili were purified by preparative isoelectric focusing to apparent homogeneity as judged by the presence of a single band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; the molecular weight of this component was calculated to be 12 60...
متن کاملAttachment pili from enterotoxigenic Escherichia coli pathogenic for humans.
Pili from enterotoxigenic Escherichia coli pathogenic for humans have been isolated by adsorption to the surface of erythrocytes followed by thermal elution. The pili are composed of two protein subunits with molecular weights of 13,100 and 12,500 as determined by sodium dodecyl sulfate-gel electrophoresis. These pili also bind to human buccal cells under temperature conditions (37 degrees C) w...
متن کاملAnalysis of a naturally occurring K99+ enterotoxigenic Escherichia coli strain that fails to produce K99.
A spontaneously occurring field isolate of enterotoxigenic Escherichia coli that was genotypically K99+ but phenotypically K99- was analyzed for the reason that it did not express K99. The defect, which was cis active, was located within an area 5' to the first gene required for K99 biogenesis and was the result of the deletion of a single base pair.
متن کاملGenes for CS2 pili of enterotoxigenic Escherichia coli and their interchangeability with those for CS1 pili.
We have cloned and sequenced the DNA needed for production of CS2 pili in Escherichia coli K-12. The four open reading frames, cotB, cotA, cotC, and cotD, show homology with the genes needed for production of CS1 and CFA/I pili, which are also found on enterotoxigenic E. coli associated with human diarrheal disease. We also report that CotA plus CotB interact with the CS1 gene products CooC and...
متن کاملIsolation and structural characterization of the equine erythrocyte receptor for enterotoxigenic Escherichia coli K99 fimbrial adhesin.
The erythrocyte receptor for Escherichia coli K99 fimbrial adhesin was isolated from equine erythrocytes and characterized as Neu5Gc-alpha(2----3)-Galp-beta(1----4)-GLcp-beta(1----1)-Ceramide. This glycolipid acted as the receptor for K99 by four different experimental approaches: inhibition of equine erythrocyte hemagglutination by preincubation of K99-positive bacteria or purified K99 fimbria...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1983
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-129-6-1975